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KMID : 0545119980080030203
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 3 p.203 ~ p.207
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Invertase Production by Fed-batch Fermentations of Recombinant Saccharomyces cerevisiae
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Koo, Ja Hyup
Kim, Sang Yong/Park, Yong Cheol/Han, Soo Nam/Seo, Jin Ho
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Abstract
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Fed-batch fermentations with different feeding media were carried out in order to increase the productivity of invertase expression using a recombinant Saccharomyces cerevisiae containing plasmid pRB58. Two hatch cultures showed the expression of the SUC2 gene at a low concentration of glucose, suggesting that glucose concentration could be used as a control variable in a fed-batch operation mode. In the fed-batch culture by feeding the basal medium, cell mass and specific invertase activity did not increase much as compared with the simple batch culture. A series of fed-batch cultures revealed that the sucrose-supplemented medium increased cell mass whereas the enriched medium did specific invertase activity. To capitalize on the synergism of the sucrose-supplemented medium and the enriched medium, the sucrose-supplemented enriched medium was used as a feeding medium. The fed-batch culture using this medium resulted in a 2.4-fold increase in cell mass and a 1.9-fold enhancement in specific invertase activity compared with those of the hatch culture. The increase in cell mass and specific invertase activity led to a marked increase in total invertase activity, 250 U/㎖, which was 6.3 times higher than that of the batch culture.
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